Hotspot

petA-psbJ

LSC · 62436-63445 · 1010 bp

Back to Salicornia

Pi 0.0121

MarkerSeek score 65.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 26
pi: 0.0121
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCGTACAAGGTCTTTTGTT	ACTGGTATTGTTGTGATCGGT	45.0	42.9	57.0	57.3	1139-1202 bp	1.0	64.0
petA-psbJ_p2	CGCGTACAAGGTCTTTTGTTC	ACTGGTATTGTTGTGATCGGT	47.6	42.9	58.1	57.3	1139-1202 bp	1.0	63.4
petA-psbJ_p3	CGCGTACAAGGTCTTTTGTT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.0	56.2	1184-1247 bp	1.0	63.1
petA-psbJ_p4	CGAGAAGGTTCAATTGTCCG	ACTGGTATTGTTGTGATCGGT	50.0	42.9	56.8	57.3	1062-1125 bp	1.0	63.0
petA-psbJ_p5	CGCGTACAAGGTCTTTTGTT	CTGGATTAGGCTCATCACTGT	45.0	47.6	57.0	57.2	1087-1150 bp	1.0	62.9