Hotspot

psaA-ycf3

LSC · 41187-41944 · 758 bp

Back to Salicornia

Pi 0.0245

MarkerSeek score 57.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 43
pi: 0.0245
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TAATGGAACCAACCCGCAAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	1389-1468 bp	1.0	82.6
psaA-ycf3_p2	TAATGGAACCAACCCGCAAA	TTGCGGAAAATACGACGGAT	45.0	45.0	58.0	58.0	1744-1780 bp	0.8	72.5
psaA-ycf3_p3	GGCACTAGGTCCAATGTGAG	TTGCGGAAAATACGACGGAT	55.0	45.0	58.0	58.0	1547-1583 bp	0.8	72.4
psaA-ycf3_p4	GCATCAGCATGTAGGTTCCA	TTGCGGAAAATACGACGGAT	50.0	45.0	58.0	58.0	1369-1405 bp	0.8	72.4
psaA-ycf3_p5	CGCTCGTATGGCTATCGAAA	TTGCGGAAAATACGACGGAT	50.0	45.0	58.0	58.0	1395-1431 bp	0.8	72.3