Hotspot

ACI62_gt010-psbD

LSC · 30817-32140 · 1324 bp

Back to Salicornia

Pi 0.00698

MarkerSeek score 62.40

Primer available yes

Label ACI62_gt010-psbD

Feature Metadata

label_name: ACI62_gt010-psbD
feature_type: igs
region: LSC
length_bp: 1324
variable_sites: 21
pi: 0.00698
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ACI62_gt010-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1445-1489 bp	1.0	81.4
ACI62_gt010-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1448-1492 bp	1.0	81.2
ACI62_gt010-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1444-1488 bp	1.0	80.2
ACI62_gt010-psbD_p4	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1447-1491 bp	1.0	80.1
ACI62_gt010-psbD_p5	ACATAGGGACTCAGCCAAGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.7	58.2	1492-1536 bp	1.0	79.8