Hotspot

ACI62_gt008-ACI62_gt009

LSC · 30102-30165 · 64 bp

Back to Salicornia

Pi 0.0375

MarkerSeek score 60.20

Primer available yes

Label ACI62_gt008-ACI62_gt009

Feature Metadata

label_name: ACI62_gt008-ACI62_gt009
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 5
pi: 0.0375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ACI62_gt008-ACI62_gt009_p1	GATGAGCGAATTTTTGGGCC	TAGCGGTTCAGGACATCTCT	50.0	50.0	58.1	57.9	223 bp	1.0	80.0
ACI62_gt008-ACI62_gt009_p2	GATGAGCGAATTTTTGGGCC	TCTAGCGGTTCAGGACATCT	50.0	50.0	58.1	57.9	225 bp	1.0	80.0
ACI62_gt008-ACI62_gt009_p3	GATGAGCGAATTTTTGGGCC	GTCTAGCGGTTCAGGACATC	50.0	55.0	58.1	57.8	226 bp	1.0	79.5
ACI62_gt008-ACI62_gt009_p4	GATGAGCGAATTTTTGGGCC	CAAGGAGACAACGGGGATTC	50.0	55.0	58.1	58.3	199 bp	1.0	79.3
ACI62_gt008-ACI62_gt009_p5	CCAGCGTAGACATATTGCCA	TAGCGGTTCAGGACATCTCT	50.0	50.0	57.8	57.9	189 bp	1.0	79.0