Hotspot

ACI62_gt003-ACI62_gt004

LSC · 7612-8750 · 1139 bp

Back to Salicornia

Pi 0.0409

MarkerSeek score 82.20

Primer available yes

Label ACI62_gt003-ACI62_gt004

Feature Metadata

label_name: ACI62_gt003-ACI62_gt004
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 105
pi: 0.0409
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ACI62_gt003-ACI62_gt004_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	1262-1384 bp	1.0	60.8
ACI62_gt003-ACI62_gt004_p2	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	1265-1387 bp	1.0	59.7
ACI62_gt003-ACI62_gt004_p3	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	1264-1386 bp	1.0	59.7
ACI62_gt003-ACI62_gt004_p4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	1263-1385 bp	1.0	58.9
ACI62_gt003-ACI62_gt004_p5	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	1248-1370 bp	1.0	57.1