Hotspot

rps16-ACI62_gt002

LSC · 5679-6273 · 595 bp

Back to Salicornia

Pi 0.0177

MarkerSeek score 62.20

Primer available yes

Label rps16-ACI62_gt002

Feature Metadata

label_name: rps16-ACI62_gt002
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 23
pi: 0.0177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-ACI62_gt002_p1	AAGAAATCCGGGTTCGAGTG	TAGGCTTCCGGTAATTTGGC	50.0	50.0	57.9	58.0	1195-1226 bp	1.0	84.8
rps16-ACI62_gt002_p2	AAGAAATCCGGGTTCGAGTG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	1282-1313 bp	1.0	84.7
rps16-ACI62_gt002_p3	CCACAACGGATCATGTCCTT	TAGGCTTCCGGTAATTTGGC	50.0	50.0	57.9	58.0	1113-1144 bp	1.0	84.7
rps16-ACI62_gt002_p4	CTAGACCCCTTCCCCCTAAG	TAGGCTTCCGGTAATTTGGC	60.0	50.0	57.9	58.0	1280-1311 bp	0.8	74.1
rps16-ACI62_gt002_p5	CTAGACCCCTTCCCCCTAAG	CTTACAGCAGCTTGCCAAAC	60.0	50.0	57.9	57.9	1367-1398 bp	0.8	73.8