Hotspot

ACI62_gt001-rps16

LSC · 3975-4545 · 571 bp

Back to Salicornia

Pi 0.0279
MarkerSeek score 64.40
Primer available yes
Label ACI62_gt001-rps16

Feature Metadata

label_name
ACI62_gt001-rps16
feature_type
igs
region
LSC
length_bp
571
variable_sites
38
pi
0.0279
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ACI62_gt001-rps16_p1 AAAGCCGAGTACTCTACCGT AGAAGGCGGGGGTTTTTAAC 50.0 50.0 58.2 58.4 628-677 bp 1.0 83.7
ACI62_gt001-rps16_p2 AAAGCCGAGTACTCTACCGT GAAGGCGGGGGTTTTTAACT 50.0 50.0 58.2 58.4 627-676 bp 1.0 83.7
ACI62_gt001-rps16_p3 AAGCCGAGTACTCTACCGTT AGAAGGCGGGGGTTTTTAAC 50.0 50.0 58.2 58.4 627-676 bp 1.0 83.7
ACI62_gt001-rps16_p4 AAGCCGAGTACTCTACCGTT GAAGGCGGGGGTTTTTAACT 50.0 50.0 58.2 58.4 626-675 bp 1.0 83.7
ACI62_gt001-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAGGCGGGGGTTTTTAA 50.0 45.0 58.2 57.6 628-677 bp 1.0 83.6