Hotspot

ndhA

SSC · 125272-127567 · 2296 bp

Back to Salacia

Pi 0.0066

MarkerSeek score 62.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2296
variable_sites: 22
pi: 0.0066
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCCAGTCACCATAGGGAG	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.4	57.9	2390-2415 bp	1.0	77.4
ndhA_p2	GAACCCAGTCACCATAGGGA	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.4	57.9	2391-2416 bp	1.0	77.4
ndhA_p3	AACCCAGTCACCATAGGGAG	GGGGGAAGTTGATCGTTGAA	55.0	50.0	58.4	57.8	2388-2413 bp	1.0	76.9
ndhA_p4	GAACCCAGTCACCATAGGGA	GGGGGAAGTTGATCGTTGAA	55.0	50.0	58.4	57.8	2389-2414 bp	1.0	76.9
ndhA_p5	CTGAACCCAGTCACCATAGG	ATGGGGGAAGTTGATCGTTG	55.0	50.0	57.3	57.9	2393-2418 bp	1.0	75.2