Hotspot

trnP-UGG-psaJ

LSC · 68922-69336 · 415 bp

Back to Salacia

Pi 0.0253

MarkerSeek score 65.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 14
pi: 0.0253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAGTGCTTAATACCGGTGCC	45.0	50.0	57.9	58.3	481-500 bp	1.0	82.4
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAGTGCTTAATACCGGTGCC	45.0	50.0	58.2	58.3	482-501 bp	1.0	81.9
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAGTGCTTAATACCGGTGCC	45.0	50.0	57.8	58.3	489-508 bp	1.0	81.7
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	GCCGCGGAGAGATATGTTTT	45.0	50.0	57.9	58.4	464-483 bp	1.0	81.3
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	GCCGCGGAGAGATATGTTTT	45.0	50.0	58.2	58.4	465-484 bp	1.0	80.8