Hotspot

trnM-CAU-atpE

LSC · 54945-55230 · 286 bp

Back to Salacia

Pi 0.042

MarkerSeek score 63.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 18
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	58.4	58.0	412 bp	1.0	80.8
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	TTGAGGCAAATCTCGCTCTC	50.0	50.0	58.4	58.0	400 bp	1.0	80.8
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	385 bp	1.0	80.8
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	384 bp	1.0	79.0
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	57.3	58.0	413 bp	1.0	78.8