Hotspot

trnT-UGU-trnL-UAA

LSC · 48693-49805 · 1113 bp

Back to Salacia

Pi 0.00549

MarkerSeek score 61.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 9
pi: 0.00549
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTCTAACCGCTGAGCTAAGC	CGTACCAAGGCTCAATCCAA	55.0	50.0	58.2	57.9	1163-1194 bp	1.0	80.9
trnT-UGU-trnL-UAA_p2	CTCTAACCGCTGAGCTAAGC	GTTTCCGTACCAAGGCTCAA	55.0	50.0	58.2	58.1	1168-1199 bp	1.0	80.8
trnT-UGU-trnL-UAA_p3	CTCTAACCGCTGAGCTAAGC	CCGTAGCGTCTACCAATTCC	55.0	55.0	58.2	58.2	1137-1168 bp	1.0	80.6
trnT-UGU-trnL-UAA_p4	CTCTAACCGCTGAGCTAAGC	AAGTTTCCGTACCAAGGCTC	55.0	50.0	58.2	57.8	1170-1201 bp	1.0	80.3
trnT-UGU-trnL-UAA_p5	CTCTAACCGCTGAGCTAAGC	AGTCCGTAGCGTCTACCAAT	55.0	50.0	58.2	58.2	1140-1171 bp	1.0	79.9