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atpH-atpI

LSC · 13634-14888 · 1255 bp

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Pi 0.00864

MarkerSeek score 67.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1255
variable_sites: 16
pi: 0.00864
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CCATGGAAGGGCATCATTGA	45.0	50.0	58.1	57.9	1275-1305 bp	1.0	82.0
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TCCATGGAAGGGCATCATTG	45.0	50.0	58.1	57.9	1276-1306 bp	1.0	82.0
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	CCATGGAAGGGCATCATTGA	50.0	50.0	57.8	57.9	1285-1315 bp	1.0	81.5
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TCCATGGAAGGGCATCATTG	50.0	50.0	57.8	57.9	1286-1316 bp	1.0	81.5
atpH-atpI_p5	ATAGAAGCAAGACCGACAGC	CCATGGAAGGGCATCATTGA	50.0	50.0	57.7	57.9	1310-1340 bp	1.0	80.4