Hotspot

trnS-GGU-trnG-GCC

LSC · 7900-8692 · 793 bp

Back to Salacia

Pi 0.0164

MarkerSeek score 63.80

Primer available yes

Label trnS-GGU-trnG-GCC

Feature Metadata

label_name: trnS-GGU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 19
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGU-trnG-GCC_p1	TAGCAATCCGACGCTTTAGG	TATGAATCCAACCGAGGGAC	50.0	50.0	57.8	56.7	912-928 bp	1.0	69.6
trnS-GGU-trnG-GCC_p2	TAGCAATCCGACGCTTTAGG	ATGAATCCAACCGAGGGACC	50.0	55.0	57.8	59.5	911-927 bp	1.0	68.4
trnS-GGU-trnG-GCC_p3	TAGCAATCCGACGCTTTAGG	ATATGAATCCAACCGAGGGAC	50.0	47.6	57.8	57.2	913-929 bp	1.0	65.8
trnS-GGU-trnG-GCC_p4	CGAATTAGCAATCCGACGCT	TATGAATCCAACCGAGGGAC	50.0	50.0	58.8	56.7	917-933 bp	1.0	65.1
trnS-GGU-trnG-GCC_p5	TAGCAATCCGACGCTTTAGG	TCGGAATATGAATCCAACCGAG	50.0	45.5	57.8	58.0	918-934 bp	1.0	63.9