primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	76	609	629-651	1.000000	83.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	76	609	643-665	1.000000	83.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	609	642-664	1.000000	83.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	609	644-666	1.000000	83.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACAATCCACTGCCTT	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	609	649-671	1.000000	83.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGGGCTCAACCTACAGAAAC	20	20	50.0	55.0	58.2	58.2	20.3	0.0	0.345914	4498	5089	652-747	1.000000	82.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGGGCTCAACCTACAGAAAC	20	20	50.0	55.0	58.2	58.2	20.3	0.0	0.345914	4498	5089	651-746	1.000000	82.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	GGGGCTCAACCTACAGAAAC	20	20	50.0	55.0	57.5	58.2	0.0	0.0	0.638251	4498	5089	638-733	1.000000	80.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCACTTAAAAGCCGAG	GGGGCTCAACCTACAGAAAC	20	20	50.0	55.0	58.6	58.2	6.4	0.0	0.748031	4498	5089	663-758	1.000000	79.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCGCACTTAAAAGCCGAGTA	GGGGCTCAACCTACAGAAAC	20	20	45.0	55.0	57.3	58.2	3.0	0.0	0.906184	4498	5089	661-756	1.000000	78.1
psbI-trnS-GGU_p1	psbI-trnS-GGU	1	AATCCCGGACGCGAAGAATA	GTTCTTCGTACCGAGGGTTC	20	20	50.0	55.0	59.3	58.0	15.9	0.0	1.263486	7574	7812	282-296	1.000000	76.4
psbI-trnS-GGU_p2	psbI-trnS-GGU	2	ATCCCGGACGCGAAGAATAA	GTTCTTCGTACCGAGGGTTC	20	20	50.0	55.0	59.3	58.0	15.9	0.0	1.263486	7574	7812	281-295	1.000000	76.4
psbI-trnS-GGU_p3	psbI-trnS-GGU	3	ATCCCGGACGCGAAGAATAA	CCTAAAGCGTCGGATTGCTA	20	20	50.0	50.0	59.3	57.8	15.9	7.9	1.470503	7574	7812	314-328	1.000000	74.5
psbI-trnS-GGU_p4	psbI-trnS-GGU	4	AATCCCGGACGCGAAGAATA	ATTCGGAGAGATGGCTGAGT	20	20	50.0	50.0	59.3	58.2	15.9	0.0	1.464613	7574	7812	337-351	1.000000	74.4
psbI-trnS-GGU_p5	psbI-trnS-GGU	5	ATCCCGGACGCGAAGAATAA	ATTCGGAGAGATGGCTGAGT	20	20	50.0	50.0	59.3	58.2	15.9	0.0	1.464613	7574	7812	336-350	1.000000	74.4
trnS-GGU-trnG-GCC_p1	trnS-GGU-trnG-GCC	1	TAGCAATCCGACGCTTTAGG	TATGAATCCAACCGAGGGAC	20	20	50.0	50.0	57.8	56.7	0.0	0.0	1.512140	7900	8692	912-928	1.000000	69.6
trnS-GGU-trnG-GCC_p2	trnS-GGU-trnG-GCC	2	TAGCAATCCGACGCTTTAGG	ATGAATCCAACCGAGGGACC	20	20	50.0	55.0	57.8	59.5	0.0	0.0	1.672355	7900	8692	911-927	1.000000	68.4
trnS-GGU-trnG-GCC_p3	trnS-GGU-trnG-GCC	3	TAGCAATCCGACGCTTTAGG	ATATGAATCCAACCGAGGGAC	20	21	50.0	47.6	57.8	57.2	0.0	0.0	1.990530	7900	8692	913-929	1.000000	65.8
trnS-GGU-trnG-GCC_p4	trnS-GGU-trnG-GCC	4	CGAATTAGCAATCCGACGCT	TATGAATCCAACCGAGGGAC	20	20	50.0	50.0	58.8	56.7	0.0	0.0	2.084059	7900	8692	917-933	1.000000	65.1
trnS-GGU-trnG-GCC_p5	trnS-GGU-trnG-GCC	5	TAGCAATCCGACGCTTTAGG	TCGGAATATGAATCCAACCGAG	20	22	50.0	45.5	57.8	58.0	0.0	0.0	2.228861	7900	8692	918-934	1.000000	63.9
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	CCATGGAAGGGCATCATTGA	20	20	45.0	50.0	58.1	57.9	1.4	5.6	0.179987	13634	14888	1275-1305	1.000000	82.0
atpH-atpI_p2	atpH-atpI	2	AACAGAAGCGGCAGAAATCA	TCCATGGAAGGGCATCATTG	20	20	45.0	50.0	58.1	57.9	1.4	13.5	0.179987	13634	14888	1276-1306	1.000000	82.0
atpH-atpI_p3	atpH-atpI	3	CAGCAGCAATAACAGAAGCG	CCATGGAAGGGCATCATTGA	20	20	50.0	50.0	57.8	57.9	0.0	5.6	0.245870	13634	14888	1285-1315	1.000000	81.5
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	TCCATGGAAGGGCATCATTG	20	20	50.0	50.0	57.8	57.9	0.0	13.5	0.245870	13634	14888	1286-1316	1.000000	81.5
atpH-atpI_p5	atpH-atpI	5	ATAGAAGCAAGACCGACAGC	CCATGGAAGGGCATCATTGA	20	20	50.0	50.0	57.7	57.9	0.0	5.6	0.377784	13634	14888	1310-1340	1.000000	80.4
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.7	57.8	0.0	1.1	0.446564	37510	38246	796-1019	1.000000	81.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	12.2	1.1	0.462726	37510	38246	785-1008	1.000000	81.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	57.6	57.8	3.7	1.1	0.535240	37510	38246	786-1009	1.000000	81.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CCTGAGGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.7	57.8	0.0	1.1	0.906477	37510	38246	787-1010	1.000000	78.0
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GAGGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.2	57.8	12.2	1.1	0.995693	37510	38246	784-1007	1.000000	77.3
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AAGCCGCCGAATATTGGAAA	20	20	50.0	45.0	57.9	58.2	4.1	0.0	0.357660	43619	44313	862-870	1.000000	78.5
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.9	57.6	4.1	18.7	0.471060	43619	44313	839-847	1.000000	77.7
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTTGAGAAATGGCCGG	AAGCCGCCGAATATTGGAAA	20	20	50.0	45.0	58.5	58.2	0.0	0.0	0.708693	43619	44313	887-895	1.000000	75.7
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	GATCAAGCCGCCGAATATTG	20	20	50.0	50.0	57.9	57.4	4.1	0.0	0.716823	43619	44313	866-874	1.000000	75.6
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	AAGCCGCCGAATATTGGAAA	20	20	50.0	45.0	58.5	58.2	0.0	0.0	0.727236	43619	44313	815-823	1.000000	75.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CTCTAACCGCTGAGCTAAGC	CGTACCAAGGCTCAATCCAA	20	20	55.0	50.0	58.2	57.9	13.7	0.0	0.261409	48693	49805	1163-1194	1.000000	80.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CTCTAACCGCTGAGCTAAGC	GTTTCCGTACCAAGGCTCAA	20	20	55.0	50.0	58.2	58.1	13.7	0.0	0.266572	48693	49805	1168-1199	1.000000	80.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CTCTAACCGCTGAGCTAAGC	CCGTAGCGTCTACCAATTCC	20	20	55.0	55.0	58.2	58.2	13.7	10.2	0.304362	48693	49805	1137-1168	1.000000	80.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CTCTAACCGCTGAGCTAAGC	AAGTTTCCGTACCAAGGCTC	20	20	55.0	50.0	58.2	57.8	13.7	0.0	0.333891	48693	49805	1170-1201	1.000000	80.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CTCTAACCGCTGAGCTAAGC	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	58.2	58.2	13.7	10.2	0.393267	48693	49805	1140-1171	1.000000	79.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGATAGGGTG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	50881	51764	997-1032	1.000000	83.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGATAGGGTGG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	50881	51764	996-1031	1.000000	83.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GCATTGAGAATGGTCGGGAT	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.065515	50881	51764	1012-1047	1.000000	83.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GCATTGAGAATGGTCGGGAT	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.065515	50881	51764	1011-1046	1.000000	83.5
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	50881	51764	960-995	1.000000	83.5
ndhJ-ndhK_p1	ndhJ-ndhK	1	GAGCAGACAAACAACCTTGC	ACACAGCAGGGTCAGTATCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.181284	52242	52375	170-203	1.000000	84.3
ndhJ-ndhK_p2	ndhJ-ndhK	2	TGAACTAGCCCGTGTTTGAC	ACACAGCAGGGTCAGTATCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.182125	52242	52375	195-228	1.000000	84.1
ndhJ-ndhK_p3	ndhJ-ndhK	3	TCCGCGGGCTTTATTTGTAA	ACACAGCAGGGTCAGTATCT	20	20	45.0	50.0	57.9	58.1	27.8	0.0	0.166724	52242	52375	252-285	1.000000	83.9
ndhJ-ndhK_p4	ndhJ-ndhK	4	CCTTGGTAATCGAAGCCCAA	ACACAGCAGGGTCAGTATCT	20	20	50.0	50.0	57.9	58.1	11.2	0.0	0.178606	52242	52375	222-255	1.000000	83.9
ndhJ-ndhK_p5	ndhJ-ndhK	5	CAATCCGCGGGCTTTATTTG	ACACAGCAGGGTCAGTATCT	20	20	50.0	50.0	58.2	58.1	27.8	0.0	0.216910	52242	52375	255-288	1.000000	83.4
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	AGAGGCAAACAATTGAGGCA	20	20	50.0	45.0	58.4	58.0	13.2	13.6	0.411678	54945	55230	412-412	1.000000	80.8
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	TTGAGGCAAATCTCGCTCTC	20	20	50.0	50.0	58.4	58.0	13.2	0.0	0.427062	54945	55230	400-400	1.000000	80.8
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	58.4	58.0	13.2	0.0	0.442584	54945	55230	385-385	1.000000	80.8
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	20	20	50.0	55.0	58.4	58.3	13.2	0.0	0.671029	54945	55230	384-384	1.000000	79.0
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GTATTGCTTTCATACGGCGG	AGAGGCAAACAATTGAGGCA	20	20	50.0	45.0	57.3	58.0	13.2	13.6	0.663631	54945	55230	413-413	1.000000	78.8
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	AAGTGCTTAATACCGGTGCC	20	20	45.0	50.0	57.9	58.3	5.7	19.9	0.367441	68922	69336	481-500	1.000000	82.4
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	AAGTGCTTAATACCGGTGCC	20	20	45.0	50.0	58.2	58.3	5.7	19.9	0.424698	68922	69336	482-501	1.000000	81.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTGTACCCAAAACAAACGCG	AAGTGCTTAATACCGGTGCC	20	20	45.0	50.0	57.8	58.3	3.5	19.9	0.443855	68922	69336	489-508	1.000000	81.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAAACAAACGCGCTACCAAG	GCCGCGGAGAGATATGTTTT	20	20	45.0	50.0	57.9	58.4	5.7	33.5	0.530857	68922	69336	464-483	1.000000	81.3
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CAAAACAAACGCGCTACCAA	GCCGCGGAGAGATATGTTTT	20	20	45.0	50.0	58.2	58.4	5.7	33.5	0.588115	68922	69336	465-484	1.000000	80.8
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	113831	114434	742-745	1.000000	79.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	113831	114434	779-782	1.000000	78.5
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	57.8	58.1	18.9	0.0	0.311620	113831	114434	783-786	1.000000	78.3
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	20	20	55.0	50.0	57.8	57.8	18.9	15.9	0.366304	113831	114434	706-709	1.000000	78.0
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	113831	114434	693-696	1.000000	77.9
ndhA_p1	ndhA	1	AACCCAGTCACCATAGGGAG	ATGGGGGAAGTTGATCGTTG	20	20	55.0	50.0	58.4	57.9	0.0	0.0	0.544046	125272	127567	2390-2415	1.000000	77.4
ndhA_p2	ndhA	2	GAACCCAGTCACCATAGGGA	ATGGGGGAAGTTGATCGTTG	20	20	55.0	50.0	58.4	57.9	0.0	0.0	0.548711	125272	127567	2391-2416	1.000000	77.4
ndhA_p3	ndhA	3	AACCCAGTCACCATAGGGAG	GGGGGAAGTTGATCGTTGAA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.609477	125272	127567	2388-2413	1.000000	76.9
ndhA_p4	ndhA	4	GAACCCAGTCACCATAGGGA	GGGGGAAGTTGATCGTTGAA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.614142	125272	127567	2389-2414	1.000000	76.9
ndhA_p5	ndhA	5	CTGAACCCAGTCACCATAGG	ATGGGGGAAGTTGATCGTTG	20	20	55.0	50.0	57.3	57.9	0.0	0.0	0.827625	125272	127567	2393-2418	1.000000	75.2
ycf1_p1	ycf1	1	ATGAGTCCTTCGGATCTTCA	AGTACCCCAAAAATGGCGAA	20	20	45.0	45.0	55.7	57.7	0.0	0.0	2.641288	131134	131253	200-224	1.000000	61.8
ycf1_p2	ycf1	2	TGAGTCCTTCGGATCTTCAT	AGTACCCCAAAAATGGCGAA	20	20	45.0	45.0	55.7	57.7	0.0	0.0	2.641288	131134	131253	199-223	1.000000	61.8
ycf1_p3	ycf1	3	ATGAGTCCTTCGGATCTTCA	CCCCAAAAATGGCGAAATGT	20	20	45.0	45.0	55.7	57.5	0.0	0.0	2.816199	131134	131253	196-220	1.000000	60.5
ycf1_p4	ycf1	4	TGAGTCCTTCGGATCTTCAT	ACCCCAAAAATGGCGAAATG	20	20	45.0	45.0	55.7	57.5	0.0	0.0	2.816199	131134	131253	196-220	1.000000	60.5
ycf1_p5	ycf1	5	TGAGTCCTTCGGATCTTCAT	CCCCAAAAATGGCGAAATGT	20	20	45.0	45.0	55.7	57.5	0.0	0.0	2.816199	131134	131253	195-219	1.000000	60.5
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	135678	136281	742-745	1.000000	79.1
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	135678	136281	779-782	1.000000	78.5
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.1	57.8	0.0	18.9	0.311620	135678	136281	783-786	1.000000	78.3
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	15.9	18.9	0.366304	135678	136281	706-709	1.000000	78.0
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	135678	136281	693-696	1.000000	77.9