Hotspot

ycf1

SSC · 125663-131227 · 5565 bp

Back to Salacca

Pi 0.00324

MarkerSeek score 54.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 18
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTCCGGGAAGAACCAAGG	CGATACCTGGACCAATACGC	50.0	55.0	57.8	58.2	225 bp	1.0	78.6
ycf1_p2	GCACAAATTCCGGGAAGAAC	CGATACCTGGACCAATACGC	50.0	55.0	57.7	58.2	230 bp	1.0	77.5
ycf1_p3	ACAAATTCCGGGAAGAACCA	CGATACCTGGACCAATACGC	45.0	55.0	57.3	58.2	228 bp	1.0	75.0
ycf1_p4	GGCACAAATTCCGGGAAGAA	CGATACCTGGACCAATACGC	50.0	55.0	58.7	58.2	231 bp	1.0	74.3
ycf1_p5	AATTCCGGGAAGAACCAAGG	GATACCTGGACCAATACGCA	50.0	50.0	57.8	57.1	224 bp	1.0	73.2