Hotspot

rps16

LSC · 4969-6093 · 1125 bp

Back to Salacca

Pi 0.00533

MarkerSeek score 54.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1125
variable_sites: 6
pi: 0.00533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGAAAAAGGCAAATTGTGT	CCGGTTGAGCCAATGACTAT	36.4	50.0	57.1	57.7	1298-1305 bp	1.0	54.7
rps16_p2	GCAAGAAAAAGGCAAATTGTGT	GGTATACACACCGGTTGAGC	36.4	55.0	57.1	58.4	1308-1315 bp	1.0	54.5
rps16_p3	GCAAGAAAAAGGCAAATTGTGT	GTATACACACCGGTTGAGCC	36.4	55.0	57.1	58.4	1307-1314 bp	1.0	54.5
rps16_p4	AGCAAGAAAAAGGCAAATTGTG	CCGGTTGAGCCAATGACTAT	36.4	50.0	56.9	57.7	1299-1306 bp	1.0	52.5
rps16_p5	CAGCAAGAAAAAGGCAAATTGT	CCGGTTGAGCCAATGACTAT	36.4	50.0	56.9	57.7	1300-1307 bp	1.0	52.5