Hotspot

rps14

LSC · 36962-37264 · 303 bp

Back to Salacca

Pi 0.0033

MarkerSeek score 40.70

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 1
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	TCTTTGCCTACCCCCAAAAC	TTGATTGCCTCGACATCAGG	50.0	50.0	58.0	58.0	782-794 bp	1.0	82.2
rps14_p2	TCTTTGCCTACCCCCAAAAC	GTATGGGCGTGGATGTTCTT	50.0	50.0	58.0	58.0	1022-1034 bp	1.0	81.6
rps14_p3	TCTTTGCCTACCCCCAAAAC	TGCACGTGGTTCCAAGTTAA	50.0	45.0	58.0	58.0	1335-1347 bp	1.0	81.1
rps14_p4	TCTTTGCCTACCCCCAAAAC	ATGGGCTCATGAACGTACAC	50.0	50.0	58.0	58.0	930 bp	0.5	59.8
rps14_p5	AAGGTTATGAGCCTTGCGAG	ATGGGCTCATGAACGTACAC	50.0	50.0	58.0	58.0	801 bp	0.5	59.6