Hotspot

rpl16

LSC · 82186-83802 · 1617 bp

Back to Salacca

Pi 0.00658

MarkerSeek score 55.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1617
variable_sites: 10
pi: 0.00658
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCATCCTCAATATCCCTTTTGT	AGGTTGAATAAAAATTCGATTGACCA	36.4	30.8	54.8	58.1	1678-1694 bp	1.0	44.5
rpl16_p2	TCATCCTCAATATCCCTTTTGT	GGTTGAATAAAAATTCGATTGACCA	36.4	32.0	54.8	56.9	1677-1693 bp	1.0	44.5
rpl16_p3	TCATCCTCAATATCCCTTTTGTT	AGGTTGAATAAAAATTCGATTGACCA	34.8	30.8	55.6	58.1	1678-1694 bp	1.0	44.5
rpl16_p4	TTCATCCTCAATATCCCTTTTGT	AGGTTGAATAAAAATTCGATTGACCA	34.8	30.8	55.6	58.1	1679-1695 bp	1.0	44.5
rpl16_p5	TCATCCTCAATATCCCTTTTGTT	GGTTGAATAAAAATTCGATTGACCA	34.8	32.0	55.6	56.9	1677-1693 bp	1.0	44.5