Hotspot

rps15-ycf1

SSC · 125283-125662 · 380 bp

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Pi 0.0132

MarkerSeek score 61.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 380
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGAACCCTTGTTTTCT	TGGCAGTCGTTTCAGTATGT	38.1	45.0	55.5	57.2	503-510 bp	1.0	46.5
rps15-ycf1_p2	TCAACAGAACCCTTGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	503-510 bp	1.0	45.5
rps15-ycf1_p3	CAACAGAACCCTTGTTTTCTTCT	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	502-509 bp	1.0	45.5
rps15-ycf1_p4	TGAAATTCAACAGAACCCTTGT	TGGCAGTCGTTTCAGTATGT	36.4	45.0	56.4	57.2	509-516 bp	1.0	45.1
rps15-ycf1_p5	TCAACAGAACCCTTGTTTTCTT	TGGCAGTCGTTTCAGTATGT	36.4	45.0	56.3	57.2	503-510 bp	1.0	44.6