Hotspot

rpl32-trnL-UAG

SSC · 115038-115890 · 853 bp

Back to Salacca

Pi 0.00119
MarkerSeek score 60.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
853
variable_sites
1
pi
0.00119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 55.4 58.0 934-946 bp 1.0 60.1
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 55.4 58.0 935-947 bp 1.0 60.0
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 944-956 bp 1.0 59.1
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 943-955 bp 1.0 58.5
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 942-954 bp 1.0 58.5