Hotspot

ndhC-trnV-UAC

LSC · 50671-51937 · 1267 bp

Back to Salacca

Pi 0.00237

MarkerSeek score 60.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 3
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1404-1429 bp	1.0	78.3
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1398-1423 bp	1.0	77.5
ndhC-trnV-UAC_p3	CTAACCGGGGCTAAAACTCC	AGCCCAATGCAACAAAATGC	55.0	45.0	58.0	58.5	1486-1511 bp	1.0	76.1
ndhC-trnV-UAC_p4	CTAACCGGGGCTAAAACTCC	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	1403-1428 bp	1.0	75.6
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1480-1505 bp	1.0	75.3