Hotspot

ycf3

LSC · 49508-51493 · 1986 bp

Back to Sagittaria

Pi 0.000504

MarkerSeek score 58.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1986
variable_sites: 1
pi: 0.000504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CTTGATTGGACCCTCTCAGG	TATGAGATCGTTTTCCCGGG	55.0	50.0	57.3	57.1	2128 bp	1.0	67.2
ycf3_p2	CTTGATTGGACCCTCTCAGG	TTATGAGATCGTTTTCCCGGG	55.0	47.6	57.3	57.8	2129 bp	1.0	64.9
ycf3_p3	CTTGATTGGACCCTCTCAGG	ATGAGATCGTTTTCCCGGG	55.0	52.6	57.3	57.2	2127 bp	1.0	60.1
ycf3_p4	CTTGATTGGACCCTCTCAGG	ATGAGATCGTTTTCCCGGGG	55.0	55.0	57.3	59.8	2127 bp	1.0	59.8
ycf3_p5	TCTTGATTGGACCCTCTCAG	TATGAGATCGTTTTCCCGGG	50.0	50.0	56.2	57.1	2129 bp	1.0	58.8