Hotspot

rps16

LSC · 4903-8118 · 3216 bp

Back to Sagittaria

Pi 0.00156

MarkerSeek score 56.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 3216
variable_sites: 5
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	36.4	31.8	55.7	53.0	278-476 bp	1.0	40.6
rps16_p2	AAGCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	34.8	31.8	56.4	53.0	279-477 bp	1.0	40.6
rps16_p3	AGCTTGTTACGTTAAGAAGACAA	TCTTATATGCATGCTTATACGT	34.8	31.8	56.4	53.0	278-476 bp	1.0	40.6
rps16_p4	AGCTTGTTACGTTAAGAAGACA	ATCTTATATGCATGCTTATACGT	36.4	30.4	55.7	53.6	279-477 bp	1.0	40.6
rps16_p5	GCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	38.1	31.8	54.2	53.0	277-475 bp	1.0	40.6