Hotspot

rpoA

LSC · 89628-90662 · 1035 bp

Back to Sagittaria

Pi 0.00193

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1035
variable_sites: 2
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	33.3	50.0	54.1	57.9	1144 bp	1.0	40.7
rpoA_p2	AAGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	31.8	50.0	55.0	57.9	1145 bp	1.0	40.1
rpoA_p3	AGAAAAAGGAATTGCAAAGGA	ACCCCCTAAAAGAAGGCGTA	33.3	50.0	54.1	58.3	1115 bp	1.0	40.1
rpoA_p4	AGAAAAAGGAATTGCAAAGGAT	GCAACCCCTATGCCACATAA	31.8	50.0	54.8	57.9	1144 bp	1.0	40.1
rpoA_p5	AAAGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	30.4	50.0	55.7	57.9	1146 bp	1.0	40.1