Hotspot

rpl22-rps19

LSC · 95995-97187 · 1193 bp

Back to Sagittaria

Pi 0.00278

MarkerSeek score 63.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 1193
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTTGACTTCTACGCCCGATG	ACCTACTCGAACTTTTGGCG	50.0	50.0	58.0	58.2	1254-1290 bp	1.0	82.5
rpl22-rps19_p2	AACTTTGACTTCTACGCCCG	ACCTACTCGAACTTTTGGCG	50.0	50.0	58.2	58.2	1257-1293 bp	1.0	80.9
rpl22-rps19_p3	GCCCGCGAATTTGATCAATC	ACCTACTCGAACTTTTGGCG	50.0	50.0	58.2	58.2	1321-1357 bp	1.0	80.6
rpl22-rps19_p4	TGACTTCTACGCCCGATGTA	ACCTACTCGAACTTTTGGCG	50.0	50.0	58.2	58.2	1252-1288 bp	1.0	80.6
rpl22-rps19_p5	CTTTGGGCTTTGAAAGGGGA	ACCTACTCGAACTTTTGGCG	50.0	50.0	58.3	58.2	1298-1334 bp	1.0	80.2