Hotspot

psbE-petL

LSC · 75521-76709 · 1189 bp

Back to Sagittaria

Pi 0.000841
MarkerSeek score 59.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1189
variable_sites
1
pi
0.000841
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACTGACGAATAACCAACCCG AAGCAGCCAGTAGAAAACCA 50.0 45.0 57.9 57.3 1349 bp 1.0 74.1
psbE-petL_p2 ACTGACGAATAACCAACCCG AGCAGCCAGTAGAAAACCAA 50.0 45.0 57.9 57.3 1348 bp 1.0 74.1
psbE-petL_p3 AATAACCAACCCGCGATGAA AAGCAGCCAGTAGAAAACCA 45.0 45.0 57.9 57.3 1342 bp 1.0 73.9
psbE-petL_p4 AATAACCAACCCGCGATGAA AGCAGCCAGTAGAAAACCAA 45.0 45.0 57.9 57.3 1341 bp 1.0 73.9
psbE-petL_p5 AACCAACCCGCGATGAATAG AGCAGCCAGTAGAAAACCAA 50.0 45.0 58.3 57.3 1338 bp 1.0 72.1