Hotspot

accD-psaI

LSC · 69322-70613 · 1292 bp

Back to Sagittaria

Pi 0.00232

MarkerSeek score 60.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 3
pi: 0.00232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGGTTCCTTCCTTTGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1401 bp	1.0	79.8
accD-psaI_p2	CACGGGTTCCTTCCTTTGAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1407 bp	1.0	79.1
accD-psaI_p3	GTTATTTCAGCTCCACGGGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1414 bp	1.0	79.1
accD-psaI_p4	CCTCATCGTACCACGGAATC	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	1456 bp	1.0	78.9
accD-psaI_p5	GTTATTTCAGCTCCACGGGT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	1420 bp	1.0	78.5