Hotspot

trnQ-UUG-trnG-UCC

LSC · 11274-13355 · 2082 bp

Back to Sagittaria

Pi 0.00048
MarkerSeek score 57.80
Primer available yes
Label trnQ-UUG-trnG-UCC

Feature Metadata

label_name
trnQ-UUG-trnG-UCC
feature_type
igs
region
LSC
length_bp
2082
variable_sites
1
pi
0.00048
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnG-UCC_p1 GTCCAGTCATTCGGAGGTTC CATCGTTAGCTTGGAAGGCT 55.0 50.0 58.0 58.0 2229 bp 1.0 79.7
trnQ-UUG-trnG-UCC_p2 GTCCAGTCATTCGGAGGTTC CGTTAGCTTGGAAGGCTAGG 55.0 55.0 58.0 58.1 2226 bp 1.0 79.5
trnQ-UUG-trnG-UCC_p3 GAGGTTCGAATCCTTCCGTC CATCGTTAGCTTGGAAGGCT 55.0 50.0 58.1 58.0 2216 bp 1.0 79.2
trnQ-UUG-trnG-UCC_p4 GAGGTTCGAATCCTTCCGTC CGTTAGCTTGGAAGGCTAGG 55.0 55.0 58.1 58.1 2213 bp 1.0 79.0
trnQ-UUG-trnG-UCC_p5 GTCCAGTCATTCGGAGGTTC GTTAGCTTGGAAGGCTAGGG 55.0 55.0 58.0 57.7 2225 bp 1.0 77.3