Hotspot

ycf1

SSC · 121559-127096 · 5538 bp

Pi 0.0132

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 134
pi: 0.0132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCGATTTTTCATCAGCGGT	TGAACCCATTCGTCTGTCTG	42.9	50.0	58.0	57.5	180 bp	1.0	74.8
ycf1_p2	TCTCGATTTTTCATCAGCGGT	GAACCCATTCGTCTGTCTGT	42.9	50.0	58.0	57.5	179 bp	1.0	74.8
ycf1_p3	GTCTCGATTTTTCATCAGCGG	TGAACCCATTCGTCTGTCTG	47.6	50.0	57.7	57.5	181 bp	1.0	72.5
ycf1_p4	GTCTCGATTTTTCATCAGCGG	GAACCCATTCGTCTGTCTGT	47.6	50.0	57.7	57.5	180 bp	1.0	72.5
ycf1_p5	TCAGCGGTTGATATCTTGGAG	TGAACCCATTCGTCTGTCTG	47.6	50.0	57.5	57.5	167 bp	1.0	71.9