Hotspot

rpl16

LSC · 80434-81715 · 1282 bp

Back to Sagina

Pi 0.0169
MarkerSeek score 55.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1282
variable_sites
40
pi
0.0169
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TTGTCTCAATTCCCGTGCAA CAGGCCGTATCGATGGAAAA 45.0 50.0 58.0 58.1 1976-1995 bp 1.0 82.3
rpl16_p2 TTGTCTCAATTCCCGTGCAA TGTTGAAGGAATTGGTCGCA 45.0 45.0 58.0 58.0 2318 bp 0.25 49.6
rpl16_p3 TTTGTCTCAATTCCCGTGCA TGTTGAAGGAATTGGTCGCA 45.0 45.0 58.0 58.0 2319 bp 0.25 49.6
rpl16_p4 TCGACGATTGCTAGCTCCTA TGTTGAAGGAATTGGTCGCA 50.0 45.0 58.0 58.0 2087 bp 0.25 49.5
rpl16_p5 AACATCGCCAATACGAGCAT TGTTGAAGGAATTGGTCGCA 45.0 45.0 58.0 58.0 2108 bp 0.25 49.4