Hotspot

trnN-GUU-trnR-ACG

IRa · 127486-127899 · 414 bp

Back to Sagina

Pi 0.00686
MarkerSeek score 61.80
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
414
variable_sites
5
pi
0.00686
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 GATCGAGTCCCCTGTTGTTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 567-737 bp 1.0 79.9
trnN-GUU-trnR-ACG_p2 CCCTGTTGTTCCTCAGTAGC CAGAGGATTAGAGCACGTGG 55.0 55.0 57.9 57.8 558-728 bp 1.0 79.2
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 507-677 bp 1.0 78.9
trnN-GUU-trnR-ACG_p4 ATGAGATCGAGTCCCCTGTT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 571-741 bp 1.0 78.7
trnN-GUU-trnR-ACG_p5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 596-766 bp 1.0 78.4