Hotspot

rpl32-trnL-UAG

SSC · 111343-112016 · 674 bp

Back to Sagina

Pi 0.0529
MarkerSeek score 70.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
674
variable_sites
68
pi
0.0529
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGCGTTAAAAGCTTTTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 58.5 58.1 821-835 bp 1.0 82.3
rpl32-trnL-UAG_p2 GGCGGCGTTAAAAGCTTTTT CTCTCGCACTGCTTCCTAAG 45.0 55.0 58.5 58.1 823-837 bp 1.0 82.3
rpl32-trnL-UAG_p3 GGCGGCGTTAAAAGCTTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.5 57.9 810-824 bp 1.0 82.1
rpl32-trnL-UAG_p4 GGCGGCGTTAAAAGCTTTTT ATCCGCATAAAAGAGCTGCA 45.0 45.0 58.5 58.0 1461-1481 bp 1.0 80.2
rpl32-trnL-UAG_p5 GGCGGCGTTAAAAGCTTTTT TCCGCATAAAAGAGCTGCAT 45.0 45.0 58.5 58.0 1460-1480 bp 1.0 80.2