Hotspot

trnR-ACG-trnN-GUU

IRb · 105714-106127 · 414 bp

Back to Sagina

Pi 0.00686
MarkerSeek score 61.80
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
414
variable_sites
5
pi
0.00686
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG GATCGAGTCCCCTGTTGTTC 55.0 55.0 57.8 58.0 567-737 bp 1.0 79.9
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG CCCTGTTGTTCCTCAGTAGC 55.0 55.0 57.8 57.9 558-728 bp 1.0 79.2
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 507-677 bp 1.0 78.9
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG ATGAGATCGAGTCCCCTGTT 55.0 50.0 57.8 57.8 571-741 bp 1.0 78.7
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 55.0 50.0 57.8 57.8 596-766 bp 1.0 78.4