Hotspot

psbE-petL

LSC · 63913-65166 · 1254 bp

Back to Sagina

Pi 0.0183
MarkerSeek score 48.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1254
variable_sites
42
pi
0.0183
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CATTGCGGATATTGACCCCA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 2167-2208 bp 1.0 82.2
psbE-petL_p2 ATGGAATTCCTTGTCGGCTC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 1998-2039 bp 1.0 82.2
psbE-petL_p3 CATTGCGGATATTGACCCCA AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 2145-2186 bp 1.0 82.0
psbE-petL_p4 TCGTTCGGGTTTGATTGTGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.0 58.0 2235-2277 bp 1.0 82.0
psbE-petL_p5 TTCGGGTTTGATTGTGTCGT AGCGCTCTTAGTTCAGTTCG 45.0 50.0 58.0 58.0 2232-2274 bp 1.0 82.0