Hotspot

trnT-UGU-trnL-UAA

LSC · 45627-46357 · 731 bp

Back to Sagina

Pi 0.0253
MarkerSeek score 63.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
731
variable_sites
35
pi
0.0253
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CTCCTAGGCGGCGTATTTTT AGCCAATTAAGTCCGTAGCG 50.0 50.0 58.1 58.1 1302-1331 bp 1.0 82.2
trnT-UGU-trnL-UAA_p2 AAAACCTGACCTGTTGACCC AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.9 58.1 1501-1530 bp 1.0 81.7
trnT-UGU-trnL-UAA_p3 TTTTCTGGCGATACGAACGT AGCCAATTAAGTCCGTAGCG 45.0 50.0 57.9 58.1 1475-1504 bp 1.0 81.7
trnT-UGU-trnL-UAA_p4 TGCTTTTCTGGCGATACGAA AGCCAATTAAGTCCGTAGCG 45.0 50.0 57.9 58.1 1478-1507 bp 1.0 81.6
trnT-UGU-trnL-UAA_p5 TTGCTTTTCTGGCGATACGA AGCCAATTAAGTCCGTAGCG 45.0 50.0 57.9 58.1 1479-1508 bp 1.0 81.6