Hotspot

ycf3-trnS-GGA

LSC · 43467-44235 · 769 bp

Back to Sagina

Pi 0.0173
MarkerSeek score 66.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
769
variable_sites
25
pi
0.0173
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCTTTTTCTCCCGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 939-942 bp 1.0 80.5
ycf3-trnS-GGA_p2 GCCTCTTTTTCTCCCGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 938-941 bp 1.0 80.5
ycf3-trnS-GGA_p3 TGCCTCTTTTTCTCCCGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 960-963 bp 1.0 80.0
ycf3-trnS-GGA_p4 GCCTCTTTTTCTCCCGAAGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 959-962 bp 1.0 80.0
ycf3-trnS-GGA_p5 TGCCTCTTTTTCTCCCGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.8 57.8 910-913 bp 1.0 79.8