Hotspot

trnE-UUC-trnT-GGU

LSC · 30160-30772 · 613 bp

Back to Sagina

Pi 0.0203
MarkerSeek score 65.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
613
variable_sites
23
pi
0.0203
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 702-712 bp 1.0 82.0
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 700-710 bp 1.0 82.0
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 681-691 bp 1.0 81.6
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 679-689 bp 1.0 81.6
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 699-709 bp 1.0 81.4