Hotspot

rps2-rpoC2

LSC · 15714-15979 · 266 bp

Back to Sagina

Pi 0.022
MarkerSeek score 60.90
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
266
variable_sites
11
pi
0.022
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 ACCAAAATGAACTCCCGCTT ACGAACATTAGCGTTCCCTT 45.0 45.0 58.0 57.5 436-440 bp 1.0 79.7
rps2-rpoC2_p2 AATGAACTCCCGCTTCCATC ACGAACATTAGCGTTCCCTT 50.0 45.0 58.0 57.5 431-435 bp 1.0 79.4
rps2-rpoC2_p3 CCGCTTCCATCATCTCTTCC ACGAACATTAGCGTTCCCTT 55.0 45.0 58.1 57.5 422-426 bp 1.0 78.9
rps2-rpoC2_p4 CCCGCTTCCATCATCTCTTC ACGAACATTAGCGTTCCCTT 55.0 45.0 58.1 57.5 423-427 bp 1.0 78.8
rps2-rpoC2_p5 AAAATGAACTCCCGCTTCCA ACGAACATTAGCGTTCCCTT 45.0 45.0 57.7 57.5 433-437 bp 1.0 77.5