Hotspot

atpH-atpI

LSC · 13370-14039 · 670 bp

Back to Sagina

Pi 0.0237
MarkerSeek score 51.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
670
variable_sites
29
pi
0.0237
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CCAGTGACCCAAGAAAACGA TTTTGCAACTTTAGCCGCAG 50.0 45.0 58.0 57.9 1372-1374 bp 1.0 81.3
atpH-atpI_p2 CCAGTGACCCAAGAAAACGA TCCACAAACCATTCCGACAG 50.0 50.0 58.0 58.1 1882-1884 bp 1.0 80.8
atpH-atpI_p3 CCAGTGACCCAAGAAAACGA TACAGTTACCTCACGGGGAG 50.0 55.0 58.0 58.2 1703-1705 bp 1.0 80.6
atpH-atpI_p4 CCAGTGACCCAAGAAAACGA CGCCCTTGGGTTCCTTTTAT 50.0 50.0 58.0 58.2 1788-1790 bp 1.0 80.5
atpH-atpI_p5 CCAGTGACCCAAGAAAACGA TGTCTGGTGTGGAAGTAGGT 50.0 50.0 58.0 57.9 2014 bp 0.25 48.9