Hotspot

trnK-UUU-rps16

LSC · 4251-4901 · 651 bp

Back to Sagina

Pi 0.0242
MarkerSeek score 67.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
651
variable_sites
28
pi
0.0242
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AGAGCGGAGGTTTTTAAAGA 50.0 40.0 58.0 54.6 759-796 bp 1.0 58.0
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AGAGCGGAGGTTTTTAAAGA 50.0 40.0 58.0 54.6 760-797 bp 1.0 57.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AGAGCGGAGGTTTTTAAAGA 50.0 40.0 58.2 54.6 710-747 bp 1.0 57.3
trnK-UUU-rps16_p4 CCGATGGTGTAGACGAATCC AGAGCGGAGGTTTTTAAAGA 55.0 40.0 57.9 54.6 772-809 bp 1.0 57.1
trnK-UUU-rps16_p5 CGATGGTGTAGACGAATCCC AGAGCGGAGGTTTTTAAAGA 55.0 40.0 57.9 54.6 771-808 bp 1.0 57.1