Hotspot

trnH-GUG-psbA

LSC · 74-338 · 265 bp

Back to Sagina

Pi 0.0495
MarkerSeek score 65.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
265
variable_sites
23
pi
0.0495
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCGATTGAAGTTCCAT 55.0 45.0 58.1 57.9 318-341 bp 1.0 85.2
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCGATTGAAGTTCCAT 50.0 45.0 57.9 57.9 332-355 bp 1.0 84.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCGATTGAAGTTCCAT 50.0 45.0 57.9 57.9 331-354 bp 1.0 84.8
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCGATTGAAGTTCCAT 50.0 45.0 57.9 57.9 333-356 bp 1.0 84.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCGATTGAAGTTCCATC 55.0 50.0 58.1 57.5 317-340 bp 1.0 82.4