Hotspot

rpl16

LSC · 80172-81662 · 1491 bp

Back to Saccharum

Pi 0.00125

MarkerSeek score 48.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1491
variable_sites: 8
pi: 0.00125
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAGAAAGAAAAACCAGGGGT	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.3	56.3	1642-1649 bp	1.0	44.4
rpl16_p2	CCAGAAAGAAAAACCAGGGGT	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.3	57.3	1634-1641 bp	1.0	44.4
rpl16_p3	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.0	56.3	1645-1650 bp	0.916667	43.1
rpl16_p4	TCCAGAAAGAAAAACCAGGGG	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.0	57.3	1637-1642 bp	0.916667	42.9
rpl16_p5	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCAA	47.6	34.8	58.0	57.0	1645-1650 bp	0.916667	41.0