Hotspot

orf1_sac

LSC · 6295-6684 · 390 bp

Back to Saccharum

Pi 0.00136

MarkerSeek score 47.00

Primer available yes

Label orf1_sac

Feature Metadata

label_name: orf1_sac
feature_type: gene
region: LSC
length_bp: 390
variable_sites: 3
pi: 0.00136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf1_sac_p1	ACCAACGAACAGTTCTTACCT	CAGGATCCATTTGACACCCA	42.9	50.0	57.2	57.5	457-570 bp	1.0	65.7
orf1_sac_p2	ACCAACGAACAGTTCTTACCT	ACAGGATCCATTTGACACCC	42.9	50.0	57.2	57.5	458-571 bp	1.0	65.7
orf1_sac_p3	AACCAACGAACAGTTCTTACCT	CAGGATCCATTTGACACCCA	40.9	50.0	57.9	57.5	458-571 bp	1.0	63.2
orf1_sac_p4	ACCAACGAACAGTTCTTACCTT	CAGGATCCATTTGACACCCA	40.9	50.0	57.9	57.5	457-570 bp	1.0	63.2
orf1_sac_p5	ACCAACGAACAGTTCTTACCTT	ACAGGATCCATTTGACACCC	40.9	50.0	57.9	57.5	458-571 bp	1.0	63.1