Hotspot

atpF

LSC · 35603-36986 · 1384 bp

Back to Saccharum

Pi 0.000482

MarkerSeek score 46.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1384
variable_sites: 4
pi: 0.000482
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCGAAGTAAGTGATCG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1552-1555 bp	1.0	78.0
atpF_p2	ACGAGCGAAGTAAGTGATCG	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.8	58.2	1551-1554 bp	1.0	77.4
atpF_p3	AAGGACGAGCGAAGTAAGTG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.7	57.9	1556-1559 bp	1.0	76.6
atpF_p4	AAGGACGAGCGAAGTAAGTG	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.7	58.2	1555-1558 bp	1.0	76.0
atpF_p5	CGAGCGAAGTAAGTGATCGA	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.5	57.9	1551-1554 bp	1.0	75.8