Hotspot

rpl32-trnL-UAG

SSC · 109063-109584 · 522 bp

Back to Saccharum

Pi 0.00272

MarkerSeek score 46.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 522
variable_sites: 5
pi: 0.00272
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	617-623 bp	1.0	81.3
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	620-626 bp	1.0	80.8
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	590-596 bp	1.0	80.3
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	583-589 bp	1.0	79.9
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	604-610 bp	1.0	79.8