Hotspot

ndhC-trnV-UAC

LSC · 52741-53668 · 928 bp

Back to Saccharum

Pi 0.00209

MarkerSeek score 48.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 8
pi: 0.00209
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1053-1073 bp	1.0	79.8
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.0	57.9	1146-1166 bp	1.0	79.5
ndhC-trnV-UAC_p3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1054-1074 bp	1.0	78.0
ndhC-trnV-UAC_p4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1052-1072 bp	1.0	77.4
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.3	57.9	1145-1165 bp	1.0	77.1