Hotspot

psbM-petN

LSC · 18775-19584 · 810 bp

Back to Saccharum

Pi 0.00181

MarkerSeek score 48.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 8
pi: 0.00181
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.9	58.2	960-962 bp	1.0	54.8
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	904-906 bp	1.0	53.9
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	903-905 bp	1.0	53.1
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.7	58.2	959-961 bp	1.0	52.8
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	909-911 bp	1.0	52.5