Hotspot

trnG-GCC-trnM-CAU

LSC · 12985-13475 · 491 bp

Back to Saccharum

Pi 0.00518

MarkerSeek score 41.40

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 7
pi: 0.00518
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	AATATAGGCCGTAGCAAGCG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.9	57.8	637-665 bp	1.0	79.1
trnG-GCC-trnM-CAU_p2	AATATAGGCCGTAGCAAGCG	CAGTTTTCGGCCCTACAGAG	50.0	55.0	57.9	58.3	682-710 bp	1.0	78.7
trnG-GCC-trnM-CAU_p3	AATATAGGCCGTAGCAAGCG	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.9	58.3	652-680 bp	1.0	78.7
trnG-GCC-trnM-CAU_p4	AATATAGGCCGTAGCAAGCG	TACAGAGCGGAGTAGAGCAG	50.0	55.0	57.9	58.3	669-697 bp	1.0	78.3
trnG-GCC-trnM-CAU_p5	AATATAGGCCGTAGCAAGCG	CTACAGAGCGGAGTAGAGCA	50.0	55.0	57.9	58.3	670-698 bp	1.0	78.2