Hotspot

orf1_sac-trnQ-UUG

LSC · 6685-7122 · 438 bp

Back to Saccharum

Pi 0.00107
MarkerSeek score 48.50
Primer available yes
Label orf1_sac-trnQ-UUG

Feature Metadata

label_name
orf1_sac-trnQ-UUG
feature_type
igs
region
LSC
length_bp
438
variable_sites
2
pi
0.00107
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf1_sac-trnQ-UUG_p1 AAGGCCTGTGCTGAAACTAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 509-514 bp 0.916667 71.3
orf1_sac-trnQ-UUG_p2 AGGCCTGTGCTGAAACTAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 508-513 bp 0.916667 71.3
orf1_sac-trnQ-UUG_p3 AGTTTTAAGGCCTGTGCTGA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 515-520 bp 0.916667 71.3
orf1_sac-trnQ-UUG_p4 TTAAGGCCTGTGCTGAAACT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 511-516 bp 0.916667 71.3
orf1_sac-trnQ-UUG_p5 AGGCCTGTGCTGAAACTAAA CTCGGAGGTTCGAATCCTTC 45.0 55.0 57.3 57.8 512-517 bp 0.916667 70.2