Hotspot

matK-trnK-UUU

LSC · 3248-3900 · 653 bp

Back to Saccharum

Pi 0.00107

MarkerSeek score 46.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 4
pi: 0.00107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACGAAGTAGTGTTGACGAGA	GAGCGTCCGGTGTAATTTCA	45.0	50.0	56.3	58.3	841-845 bp	1.0	64.8
matK-trnK-UUU_p2	ACGAAGTAGTGTTGACGAGA	AGCGTCCGGTGTAATTTCAT	45.0	45.0	56.3	57.6	840-844 bp	1.0	63.9
matK-trnK-UUU_p3	ACGAAGTAGTGTTGACGAGA	ATGTTTTGACAGGAGCGTCC	45.0	50.0	56.3	58.5	853-857 bp	1.0	63.3
matK-trnK-UUU_p4	TGAAAGAGAAGTGGGTAGACG	GAGCGTCCGGTGTAATTTCA	47.6	50.0	57.1	58.3	859-863 bp	1.0	63.1
matK-trnK-UUU_p5	AGAGAAGTGGGTAGACGAAG	GAGCGTCCGGTGTAATTTCA	50.0	50.0	56.0	58.3	855-859 bp	1.0	62.7